Journal: BMC Immunology

Article Title: The effect of pneumococcal immunization on total and antigen-specific B cells in patients with severe chronic kidney disease

doi: 10.1186/s12865-019-0325-9

Figure Lengend Snippet: a . Numbers of total IgG antibody secreting cells (ASC) per 200,000 peripheral blood mononuclear cells (PBMC) pneumococcal vaccine naïve ( n = 18) and previously immunized with 23-valent pneumococcal polysaccharide vaccine (PPV23) ( n = 29) patients with severe chronic kidney disease on day 7 post-immunization with PCV13. The median is displayed counts above the upper limit of detection (200) were assigned a value of 210 (8 pneumococcal vaccine naïve and 13 previously immunized with PPV23). If no spots were detected, a value of 0.5 was assigned for statistical purposes. PPV23 naïve and PPV23 immunized each had 4 values below the limit of detection. Control wells coated with methylated human serum albumin were not displayed (all values were below the limit of detection). b . Numbers of IgG antibody secreting cells (ASC) specific for pneumococcal capsular polysaccharides of serotypes 6B or 14 per 200,000 peripheral blood mononuclear cell (PBMC) in pneumococcal vaccine naïve (n = 18) and previously immunized with 23-valent pneumococcal polysaccharide vaccine (PPV23) ( n = 31) patients with severe chronic kidney disease 7 days post-immunization with PCV13 (median is shown). If no spots were detected a value of 0.5 was assigned for statistical purposes. For serotype 6B, 9 pneumococcal vaccine naïve and 16 previously immunized with PPV23 patients had ASC below the lower limit of detection. For serotype 14, 7 pneumococcal vaccine naïve and 13 previously immunized with PPV23 patients had ASC below the lower limit of detection. Control wells coated with methylated human serum albumin were not displayed (all below the limit of detection)

Article Snippet: Multi-screen IP 96-well PVDF membrane filter plates (Millipore Canada Ltd., Etobicoke, ON, CAN) were coated with either goat anti-human IgG (20 μg/ mL) (Cedarlane, Burlington, ON, CAN), or pneumococcal capsular polysaccharides of serotypes 6B, or 14 (10 μg/ mL) (Cedarlane) conjugated with methylated human serum albumin (mHSA, 10 μg/ mL) (National Institute for Biological Standards and Control, Hertfordshire, UK).

Techniques: Control, Methylation

Journal: Medicine

Article Title: Intramuscular administration of autologous total immunoglobulin G induces immunomodulatory effects on T cells in healthy human subjects

doi: 10.1097/MD.0000000000029486

Figure Lengend Snippet: Serum levels of IgG antibodies to pneumococcal capsular polysaccharide antigens (A), influenza antigens (B), house dust mite antigens (C), and extractable nuclear antigens (D) in 13 healthy human subjects who received 8 intramuscular administrations of 50 mg autologous total IgG twice a week for 4 wk (from week 0 to week 4) during the 12-wk study period. Each symbol and connecting line in the graphs represents an individual healthy subject.

Article Snippet: Serum levels of IgG antibodies to pneumococcal capsular polysaccharide antigens (GlaxoSmithKline Biologicals, Rixensart, Belgium), influenza antigens (GlaxoSmithKline, Dresden, Germany), house dust mite antigens (whole body extract of Dermatophagoides farinae , HollisterStier, Spokane, WA), and bovine thymus extract (Immunovision, Springdale, AR), as extractable nuclear antigens, were measured using ELISA as previously described.

Techniques:

Journal: Clinical and Experimental Immunology

Article Title: 10‐valent pneumococcal non‐typeable Haemophilus influenzae protein‐D conjugate vaccine (PHiD‐CV) induces memory B cell responses in healthy Kenyan toddlers

doi: 10.1111/cei.12637

Figure Lengend Snippet: Induction of memory B cells by pneumococcal non‐typeable Haemophilus influenzae protein‐D conjugate vaccine (PHiD‐CV). Frequencies of antigen‐specific memory B cells against various pneumococcal capsular polysaccharides, tetanus toxoid (TT) and diphtheria toxoid (DT) are shown in scatter dot‐plots. Horizontal line represents median. Statistical test used: Wilcoxon's signed‐rank test. P ‐values < 0·05 were considered significant. Immunoglobulin (Ig)G ASC/1M PBMCs, IgG antibody‐secreting cells per 1 million peripheral blood mononuclear cells (PBMCs).

Article Snippet: ELISPOT multi‐screen filter plates (Millipore, Darmstadt, Germany) were coated overnight at 4°C with either pneumococcal capsular polysaccharides for serotypes 1 (20 μg/ml), 6B (10 μg/ml), 14 (10 μg/ml), 19F (20 μg/ml) and 23F (20 μg/ml) (donation from GSK Biologicals, Rixensart, Belgium) in the presence of 10 μg/ml (1 and 14) or 20 μg/ml (6B, 19F and 23F) methylated human serum albumin (mHSA).

Techniques:

Journal: Clinical and Experimental Immunology

Article Title: 10‐valent pneumococcal non‐typeable Haemophilus influenzae protein‐D conjugate vaccine (PHiD‐CV) induces memory B cell responses in healthy Kenyan toddlers

doi: 10.1111/cei.12637

Figure Lengend Snippet: Relationship between plasma antibodies and memory B cells. Correlations between plasma antibodies and frequencies of memory B cells against various pneumococcal capsular polysaccharides at different time‐points are shown (top panel –day 30; bottom panel –day 210). Statistical test: Spearman's correlations. P ‐values < 0·05 were considered significant. Immunoglobulin (Ig)G antibody‐secreting cells (ASC)/1 M peripheral blood mononuclear cells (PBMCs), IgG antibody‐secreting cells per 1 million PBMCs.

Article Snippet: ELISPOT multi‐screen filter plates (Millipore, Darmstadt, Germany) were coated overnight at 4°C with either pneumococcal capsular polysaccharides for serotypes 1 (20 μg/ml), 6B (10 μg/ml), 14 (10 μg/ml), 19F (20 μg/ml) and 23F (20 μg/ml) (donation from GSK Biologicals, Rixensart, Belgium) in the presence of 10 μg/ml (1 and 14) or 20 μg/ml (6B, 19F and 23F) methylated human serum albumin (mHSA).

Techniques: Clinical Proteomics

Journal: Clinical and Experimental Immunology

Article Title: 10‐valent pneumococcal non‐typeable Haemophilus influenzae protein‐D conjugate vaccine (PHiD‐CV) induces memory B cell responses in healthy Kenyan toddlers

doi: 10.1111/cei.12637

Figure Lengend Snippet: Relationship between plasma antibodies and memory B cells. Number and proportion of participants who attained plasma immunoglobulin (Ig)G ≥ 0·35 μg/ml and/or had detectable memory B cells against various pneumococcal capsular polysaccharides at different time‐points are shown (top panel: day 30; bottom panel: day 210). Statistical test: Fisher's exact test. P ‐values < 0·05 were considered significant. Enzyme‐linked immunosorbent assay (ELISA) + : plasma IgG ≥ 0·35 μg/ml. Enzyme‐linked immunospot (ELISPOT) + : antigen‐specific response ≥ 2 times of background response and ≥ 5 antibody‐secreting cells (ASC)/1 M peripheral blood mononuclear cells (PBMCs).

Article Snippet: ELISPOT multi‐screen filter plates (Millipore, Darmstadt, Germany) were coated overnight at 4°C with either pneumococcal capsular polysaccharides for serotypes 1 (20 μg/ml), 6B (10 μg/ml), 14 (10 μg/ml), 19F (20 μg/ml) and 23F (20 μg/ml) (donation from GSK Biologicals, Rixensart, Belgium) in the presence of 10 μg/ml (1 and 14) or 20 μg/ml (6B, 19F and 23F) methylated human serum albumin (mHSA).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot

Journal: Medicine

Article Title: Intramuscular administration of autologous total immunoglobulin G induces immunomodulatory effects on T cells in healthy human subjects

doi: 10.1097/MD.0000000000029486

Figure Lengend Snippet: Serum levels of IgG antibodies to pneumococcal capsular polysaccharide antigens (A), influenza antigens (B), house dust mite antigens (C), and extractable nuclear antigens (D) in 13 healthy human subjects who received 8 intramuscular administrations of 50 mg autologous total IgG twice a week for 4 wk (from week 0 to week 4) during the 12-wk study period. Each symbol and connecting line in the graphs represents an individual healthy subject.

Article Snippet: Serum levels of IgG antibodies to pneumococcal capsular polysaccharide antigens (GlaxoSmithKline Biologicals, Rixensart, Belgium), influenza antigens (GlaxoSmithKline, Dresden, Germany), house dust mite antigens (whole body extract of Dermatophagoides farinae , HollisterStier, Spokane, WA), and bovine thymus extract (Immunovision, Springdale, AR), as extractable nuclear antigens, were measured using ELISA as previously described.

Techniques: